Novikova AA, Podlasova EYu, Grechishkina OS.

Animal Husbandry and Fodder Production. 2025. Vol. 108. No. 2. Р. 186-194.

doi:10.33284/2658-3135-108-2-186

 

Original article

Analysis of genetic diversity of Нordeum Vulgare L. adapted to the conditions of Orenburg region according to Ppd and Vrn gene alleles

 

Antonina A Novikova1, Ekaterina Yu Podlasova2, Olga S Grechishkina3

1,2,3Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1tony-novikova@yandex.ru, https://orcid.org/0000-0002-6947-9262

2katerina.pryakhina@mail.ru, https://orcid.org/0000-0002-2985-198X

3vip.olga1979@gmail.com, https://orcid.org/0000-0002-4054-3048

 

Abstract. The duration of the growing season and the form of cereal crops are mainly controlled by the loci of two genes – Ppd (photoperiodic sensitivity) and Vrn (duration of the "germination–earing" period). Barley varieties with the dominant allele Ppd-H1 are ahead of other genotypes in the rate of development (heading period) and are earlier ripening when grown under long-daylight conditions. The aim of our study was to assess the genetic diversity of spring barley varieties by the genes of vernalization - Vrn and photoperiodism - Ppd. The material for the study was 72 samples of spring barley (Hordeum vulgare) from the collection nursery and the nursery of the competitive testing laboratory of the spring barley breeding laboratory of the Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences. DNA analysis was performed on ethylated seedlings using a commercial reagent kit for the extraction of genomic DNA. Primers amplifying alleles of the Ppd and Vrn genes were selected based on literature data. Among the studied varieties, 2 samples were carriers of the Ppd gene alleles, and 4 samples were carriers of the Vrn gene alleles. The use of allele-specific markers of the Ppd and Vrn genes can significantly increase the efficiency of selection for early maturity and accelerate the selection of varieties for this trait.

Key words: spring barley, Hordeum vulgare, PCR-analysis, molecular markers, vegetation period, yield, early maturity, photoperiod, vernalization

Acknowledgments: the work was performed in accordance to the plan of research works for 2022-2026 FSBRI FRC BST RAS (No. FNWZ-2022-0015).

For citation: Novikova AA, Podlasova EYu, Grechishkina OS. Analysis of genetic diversity of Нordeum Vulgare L. adapted to the conditions of Orenburg region according to Ppd and Vrn gene alleles. Animal Husbandry and Fodder Production. 2025;108(2):186-194. (In Russ.). https://doi.org/10.33284/2658-3135-108-2-186

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Информация об авторах:

Антонина Александровна Новикова, кандидат сельскохозяйственных наук, заведующий лабораторией селекционно-генетических исследований в растениеводстве, Федеральный научный центр биологических систем и агротехнологий Российский академии наук, 460051, г. Оренбург, пр. Гагарина, 27/1, тел.: 89228884481.

Екатерина Юрьевна Подласова, кандидат сельскохозяйственных наук, научный сотрудник, Федеральный научный центр биологических систем и агротехнологий Российский академии наук, 460051, г. Оренбург, пр. Гагарина, 27/1, тел.: 89877866593.

Ольга Сергеевна Гречишкина, кандидат сельскохозяйственных наук, заведующий лабораторией селекции ярового ячменя, Федеральный научный центр биологических систем и агротехнологий Российский академии наук, 460051, г. Оренбург, пр. Гагарина, 27/1, тел.: 89225314123.

 

Information about the authors:

Antonina A Novikova, Cand. Sci. (Agriculture), Head of the Laboratory for Selection and Genetic Research in Plant Growing, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 27/1 Gagarin Ave., Orenburg, 460051, tel.: 89228884481.

Ekaterina Yu Podlasova, Cand. Sci. (Agriculture), Research Assistant, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 27/1 Gagarin Ave., Orenburg, 460051, tel.: 89877866593.

Olga S Grechishkina, Cand. Sci. (Agriculture), Head of the Laboratory for Spring Barley Breeding, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 27/1 Gagarin Ave., Orenburg, 460051, tel.: 89225314123.

The article was submitted 15.05.2025; approved after reviewing 21.05.2025; accepted for publication 16.06.2025.

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