Zelenchenkova АА.

Animal Husbandry and Fodder Production. 2025. Vol. 108. No. 2. Р. 45-59.

doi: 10.33284/2658-3135-108-2-45

Original article

The effect of proanthocyanidins on some blood parameters and intestinal microbiota in juvenile sterlet

 

Alena A. Zelenchenkova1

1Federal Research Center for Animal Husbandry - VIZh named after Academician LK Ernst, Moscow region, Dubrovitsy, Russia

1aly4383@mail.ru, https://orcid.org/0000-0001-8862-3648

Abstract. An effective way to enhance the protective function of the body of sturgeon fish raised in closed-loop water supply (CLWS) conditions may be the introduction of oligomeric proanthocyanidin with antioxidant, antibacterial and antiviral properties directly into the diet as a feed additive. This scientific and practical study is aimed at studying some physiological parameters of the effect of proanthocyanidin of grape seed extract (GSE) in the body of juvenile sterlet. The research tasks were to determine the dosage of injection into a complete feed, the effect on hematological parameters of blood serum and cortisol levels, as well as to evaluate the quantitative ratio of microbiocenosis of intestinal contents. As a result of the research, it was found that the initial optimal feeding rate for the active substance is 50 mg per 1 kg of complete feed. The introduction of GSE makes it possible to increase the absolute increase by 1.16 times, the hematocrit content by 1.48%, erythrocytes by 2.0%, leukocytes by 5.6%, lymphocytes by 7.2% and cortisol level by 6% in the blood serum. As a result of studies of the intestinal microbiota, 18 groups of microorganisms were identified in the amount of 102 – 1010 CFU/ml, while Bacteroides spp. were identified as the predominant group of bacteria, and Lactobacillus spp. is noted to be higher by 1.04 (P≤0.05) lgCFU/ml in the experimental group. The total bacterial mass increased by 4.3% due to the addition of proanthocyanidins contained in GSE. Additionally, the positive dynamics of tissue regeneration in injured fish and increased pigmentation of skin color were registered. In general, the data obtained allow us to conclude that GSE has a positive effect on the body of juvenile sterlet.

Keywords: sterlet, proanthocyanidins, feeding, blood hematology, cortisol, intestinal microbiota

Acknowledgments: the work was performed in accordance to the plan of research works for 2025-2027. FSBSI L.K. Ernst Federal Research Center for Animal Husbandry (No. FGGN-2025-0006).

For citation: Zelenchenkova АА. The effect of proanthocyanidins on some blood parameters and intestinal microbiota in juvenile sterlet. Animal Husbandry and Fodder Production. 2025;108(2):45-59. (In Russ.). https://doi.org/10.33284/2658-3135-108-2-45

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Information about the authors:

Alena A Zelenchenkova, Cand. Sci. (Agriculture), Senior Researcher, Head of the Laboratory of the fundamental fundamentals of Nutrition of Agricultural Animals and Fish, Federal Research Center for Animal Husbandry – VIJ named after Academician L.K. Ernst, 60, Dubrovitsy, Moscow Region, 142132, Russia, tel.: 8(9160)96-37-83.

The article was submitted 14.03.2025; approved after reviewing 27.03.2025; accepted for publication 16.06.2025.

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