Logvinova TI, Artemyeva OA.

Animal Husbandry and Fodder Production. 2025. Vol. 108. No. 3. Р. 82-94.

doi:10.33284/2658-3135-108-3-82

 

Original article

Study of probiotic properties of bacteria of the genus Propionibacterium 

Tatyana I Logvinova1, Olga A Artemyeva2

1,2Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, Dubrovitsy, Russia

1vijmikrob@mail.ru, https: //orcig.org/0000-0001-7075-544X

2vijmikrob@mail.ru, https://orcid.org/0000-0001-7706-4182

Abstract. Today, probiotics are considered as a real alternative to antibiotics in animal husbandry. The development and use of probiotic feed additives in animal husbandry is a promising area to ensure the production of environmentally friendly products. Probiotics support the host's microbiota, help eliminate opportunistic pathogens, stimulate beneficial biochemical and bacteriological processes in the microbiota, and their metabolites promote the restoration of the intestinal epithelium and normalize its functioning. Propionic acid microorganisms are one of the promising and popular probiotics today. These microorganisms do not break down and retain their biological activity in the gastrointestinal tract, they are highly resistant to bile acids and to antibacterial substances that are used in antibiotic therapy of various pathologies. In this regard, the research article aims to study the probiotic properties of selected cultures of Propionibacterium bacteria in relation to adverse environmental factors that reflect certain conditions of the animal gastrointestinal tract in vitro. The study isolated 5 cultures of propionic acid bacteria from milk and whey, evaluated their cultural and morphological properties, and tested their biological properties, including strain resistance to pH, sodium chloride (NaCl), and bile at different concentrations. The analysis of the obtained data showed that the isolated strains of propionic acid bacteria are resistant to low acidity (pH 2.0; 3.0; 4.0), which allows them to be present in the gastrointestinal tract, concentrations of table salt from 0.5 to 7%, as well as to 0.5% and partially to 20% of bile. The isolated cultures are promising probiotic strains for use in order to correct the microflora of the gastrointestinal tract of the animal body.

Keywords: propionic acid bacteria, probiotics, gastrointestinal tract

Acknowledgments: the work was performed in accordance to the plan of research works for 2025 Federal  Research  Center for Animal Husbandry named after Academy Member LK Ernst FGGN-2024-0016 (124020200032-4).

For citation: Logvinova TI, Artemyeva OA. Study of probiotic properties of bacteria of the genus Propionibacterium. Animal Husbandry and Fodder Production. 2025;108(3):82-94. (In Russ.). https://doi.org/10.33284/2658-3135-108-3-82

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Information about the authors:

Tatyana I Logvinova, Cand. Sci. (Biology), Researcher at the Microbiology Laboratory, Federal Research Center of Animal Husbandry – VIZ named after Academician L.K. Ernst, 60 Dubrovitsy village, Podolsk City district, Moscow region, 142132, tel.: 8(4967)651133.

Olga A Artemyeva, Cand. Sci. (Biology), Leading Researcher at the Microbiology Laboratory, Federal Research Center of Animal Husbandry – VIZ named after Academician L.K. Ernst, 60 Dubrovitsy village, Podolsk City district, Moscow region, 142132, tel.: 8(4967)651133.

The article was submitted 15.07.2025; approved after reviewing 02.09.2025; accepted for publication 15.09.2025.

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