Bulanin DI, Mingazova MS, Miroshnikova EP, Kilyakova YuV, Arinzhanov AЕ.

Animal Husbandry and Fodder Production. 2025. Vol. 108. No 3. Р. 235-246.

doi:10.33284/2658-3135-108-3-235

 

Original article

Growth and hematological parameters of carp when using nanocomposite of Cu-C in feeding

 

Dmitry I Bulanin1,2, Marina S Mingazova3, Elena P Miroshnikova4, Yulia V Kilyakova5,

Azamat Е Arinzhanov 6

1,3,4,5,6Orenburg State University, Orenburg, Russia

2Federal Research Centre for Biological Systems and Agrotechnologies of the Russian Academy

of Sciences, Orenburg, Russia

1,2bulanin.dmitry@gmail.com

3ms.mingazova@gmail.com, https://orcid.org/0000-0002-2818-1312

4elenaakva@rambler.ru, https://orcid.org/0000-0003-3804-5151

5fish-ka06@mail.ru, https://orcid.org/0000-0002-2385-264X

6arin.azamat@mail.ru, https://orcid.org/0000-0001-6534-7118

 

Abstract. Nanocomposites in fish diets represent a new area of research in the field of aquaculture and hydrobionts feed production. Additional introduction of nanocomposites into feed can stimulate improvement of feed preparation absorption and improve physiological state of the organism. The article presents the results of the study on the effect of Cu-C nanocomposite (40-60 nm) in dosages of 0.2 and 0.4 ppm on growth indices and morphobiochemical parameters of blood of carp (Cyprinus carpio). The results showed that Cu-C nanocomposite had a growth-stimulating effect after 6 weeks of the experiment, reaching maximum values at 8 weeks – from 10.8% (0.4 ppm) to 15.3% (0.2 ppm). According to the blood morphology and biochemistry data, it was revealed that the drug at a dosage of 0.2 ppm led to a decrease in platelets (P≤0.01), leukocytes (P≤0.05), ALT (P≤0.05), AST (P≤0.01), iron (P≤0.05) and phosphorus (P≤0.05), with an increase in the concentration of glucose (P≤0.05), albumin (P≤0.05) and cholesterol (P≤0.01). The Cu-C nanocomposite at a dosage of 0.4 ppm had an effect on the decrease in the level of erythrocytes (P≤0.05), hematocrit (P≤0.05), hemoglobin (P≤0.01), leukocytes (P≤0.01) and iron (P≤0.01), with an increase in glucose (P≤0.05), albumin (P≤0.01), cholesterol (P≤0.001) and calcium (P≤0.05). Thus, the Cu-C nanocomposite at dosages of 0.2 and 0.4 ppm led to an improvement in protein and lipid metabolism, which positively affected the growth of the experimental fish. The best results were obtained with the introduction of Cu-C nanocomposite at a dosage of 0.2 ppm, which was reflected in a change in the concentration of ALT and AST and an improvement in enzyme activity, as well as an increase in live weight gain. The data obtained confirm the possibility of using Cu-C nanocomposite in aquaculture, but require further research to assess its long-term impact.

Keywords: aquaculture, carp, feeding, nanocomposite, copper, ultrafine particles, growth, biochemical parameters, morphological parameters

Acknowledgments: the work was supported by the Ministry of Education and Science of the Russian Federation (No. 075-15-2024-550).

For citation: Bulanin DI, Mingazova MS, Miroshnikova EP, Kilyakova YuV, Arinzhanov AЕ. Growth and hematological parameters of carp when using nanocomposite of Cu-C in feeding. Animal Husbandry and Fodder Production. 2025;108(3):235-246. (In Russ.). https://doi.org/10.33284/2658-3135-108-3-235

References

  1. Karpenko LYu, Polistovskaya PA, Enukashvili AI, Balykina AB. Analysis of the influence of copper on carbohydrate metabolism in carp. International Bulletin of Veterinary Medicine. 2020;2:162-165. doi: 10.17238/issn2072-2419.2020.2.162
  2. Mingazova MS, Miroshnikova EP, Kilyakova YuV, Arinzhanov AЕ. The effect of biologically active feed additives in the diet on the hematological parameters of fish. Animal Husbandry and Fodder Production. 2025;108(1):158-174. doi: 10.33284/2658-3135-108-1-158
  3. Miroshnikova EP, Arinzhanov AЕ,  Kilyakova YuV,  Mingazova MS. The effect of Cu-C nanocomposite on hematological parameters of carp. Bulletin of KSAU. 2025а;3:140-148. doi: 136718/1819-4036-2025-3-140-148
  4. Nikiforov NDL, Kochetkov NI, Dzhavakhiya VV, Glagoleva EV, Ginayatov NS, Nikiforov-Nikishin AL, Kartashov MI, Uvaidov MM. Influence of the probiotic preparation “Aquasporin” on fish-breeding-biological, physiological and histological indicators of rainbow trout (Oncorhynchus mykiss). Bulletin of the Kerch State Marine Technological University. 2024;3:20-32.
  5. Gurnak EE, Nalivayko MN. Role of fisheries sector in food security. Bulletin of Rostov State University of Economics (RINH). 2022;4:33-38. doi: 10.54220/v.rsue.1991-0533.2023.80.4.005
  6. Mingazova MS, Miroshnikova EP, Arinzhanov AЕ, Kilyakova YuV. Concentration of chemical elements in carp muscle tissue when biologically active substances are included in the diet. Animal Husbandry and Fodder Production. 2023;106(4):18-29. doi: 33284/2658-3135-106-4-18
  7. Makoedov AN, Matishov GG,  Ponomareva EN. World  trends  in  the use of aquatic biological resources. Vestnik of Russian Academy of Science. 2023;93(2):179-190. doi: 31857/S086958732301005X
  8. Lyubomirova VN, Romanova EM, Romanov VV, Lyubomirova ES. Efficiency parameters of the feed additive "Pravad" in rearing Russian sturgeon in industrial aquaculture. Vestnik of Ulyanovsk State Agricultural Academy. 2024;4(68):145-150. doi: 10.18286/1816-4501-2024-4-145-150
  9. Seregin SN, Gasanova KhN, Karavaeva MS, Tazetdinov RR. Development of aquaculture: world trends and possibilities of Russian production. Economy, Labor, Management in Agriculture. 2023;2(96):113-125. doi: 10.33938/232-113
  10. 2020. The State of World Fisheries and Aquaculture 2020. Sustainability in action. Rome, FAO. doi: 10.4060/ca9229ru
  11. Miroshnikova EP, Mingazova MS, Arinzhanov AЕ, Kilyakova YuV. The elemental status of carp when biologically active feed additives are introduced into the diet. Vestnik of Astrakhan State Technical University. Series: Fishing Industry. 2025b;1:75-84. doi: 10.24143/2073-5529-2025-1-75-84
  12. Abinaya M, Shanthi S, Palmy J, Al-Ghanim KA, Govindarajan M, Vaseeharan B. Exopolysaccharides-mediated ZnO nanoparticles for the treatment of aquatic diseases in freshwater fish Oreochromis mossambicus. Toxics. 2023;11(4):313. doi: 10.3390/toxics11040313
  13. Delavari NM, Gharaei A, Mirdar HJ, Davari A, Rastiannasab A. Modulatory effect of dietary copper nanoparticles and vitamin C supplementations on growth performance, hematological and immune parameters, oxidative status, histology, and disease resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry. 2022;48:33-51. doi: 10.1007/s10695-021-01036-2
  14. Gharaei A, Khajeh M, Khosravanizadeh A, Mirdar J, Fadai R. Fluctuation of  biochemical,  immunological,  and  antioxidant  biomarkers  in  the  blood of beluga (Huso huso) under effect of dietary ZnO and chitosan–ZnO NPs. Fish Physiology and Biochemistry. 2020;46:547-561. doi: 10.1007/s10695-019-00726-2
  15. Ghirmai S, Wu H, Axelsson M, Matsuhira T, Sakai H, Undeland I. Exploring how plasma- and muscle-related parameters affect trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation of fish muscle. Scientific Reports. 2022;12(1):13446.doi: 10.1038/s41598-022-16363-4
  16. Hatab MH, Rashad E, Saleh HM, El-Sayed E-S, Abu Taleb AM. Effects of dietary supplementation of myco-fabricated zinc oxide nanoparticles on performance, histological changes, and tissues Zn concentration in broiler chicks. Scientific Reports. 2022;12(1):18791. doi: 10.1038/s41598-022-22836-3
  17. Hussain SM, Naeem E, Ali S, Adrees M, Riaz D, Paray BA, Naeem A. Evaluation of growth, nutrient absorption, body composition and blood indices under dietary exposure of iron oxide nanoparticles in Common carp (Cyprinus carpio). Journal of Animal Physiology and Animal Nutrition. 2024;108(2):366-373. doi: 10.1111/jpn.13898
  18. Liang Q, Yuan M, Xu L, Lio E, Zhang F, Mou H, Secundo F. Application of enzymes as a feed additive in aquaculture. Marine Life Science & Technology. 2022;4(2):208-221. doi: 10.1007/s42995-022-00128-z
  19. Maulu S, Hasimuna OJ,  Haambiya LH, Monde C, Musuka CG, Makorwa TH, Munganga BP, Phiri KJ, Nsekanabo JD. Climate change effects on aquaculture production: Sustainability implications, mitigation, and adaptations. Frontiers in Sustainable Food Systems. 2021;5:609097. doi: 10.3389/fsufs.2021.609097
  20. Nabi N, Ahmed I, Wani GB. Hematological and serum biochemical reference intervals of rainbow trout, Oncorhynchus mykiss cultured in  Himalayan  aquaculture:  Morphology,  morphometrics  and quantification  of  peripheral blood cells. Saudi Journal of Biological Sciences. 2022;29(4):2942-2957. doi: 10.1016/j.sjbs.2022.01.019
  21. Naguib M, Mahmoud UM, Mekkawy IA, Sayed AEH. Hepatotoxic effects of silver nanoparticles on Clarias gariepinus; Biochemical, histopathological, and histochemical studies. Toxicology Reports. 2020;7:133-141. doi: 1016/j.toxrep.2020.01.002
  22. Rahman M, Paul SI, Rahman A, Haque S, Ador AA, Foysal J, Islam T, Rahman M. Suppression of streptococcosis and modulation of the gut bacteriome in nile tilapia (Oreochromis niloticus) by the marine sediment bacteria Bacillus haynesii and Advenella mimigardefordensis. Microbiology Spectrum. 2022;10(6):e0254222. doi: 10.1128/spectrum.02542-22
  23. Rolić T, Yazdani M,  Mandić S,  Distante S.  Iron  metabolism,  calcium,  magnesium and  trace  elements:  a    Biological Trace Element Research. 2025;203(4):2216-2225.  doi: 10.1007/s12011-024-04289-z
  24. Silva VF, Mouriño JLP,  Martins ML,  Carvalho  PLPF, Rodrigues ED, Gatlin 3rd DM, Griffin MJ,  Older CE,  Yamamoto FY.  Dietary supplementation of mineral nanoparticles for channel catfish (Ictalurus punctatus). Fish Physiology and Biochemistry. 2024;50(6):2225-2236. doi: 10.1007/s10695-024-01378-7
  25. Subaramaniyam U, Allimuthu RS,  Vappu S, Ramalingam D, Balan R, Paital B, Panda N, Rath PK, Ramalingam N, Sahoo DK. Effects of microplastics, pesticides and nano-materials on fish health, oxidative stress and antioxidant defense mechanism. Frontiers in Physiology. 2023;14:1217666. doi: 10.3389/fphys.2023.1217666
  26. Ullah M, Yousafzai AM, Muhammad I, Ullah SA, Zahid M, Khan MI, Khan K, Khayyam, Nayab GE, Aschner M, Alsharif KF, Alzahrani KJ, Khan H. Effect of cypermethrin on blood hematology and biochemical parameters in fresh water fish Ctenopharyngodon idella (Grass Carp). Cell and Molecular Biology. 2022;68(10-10):15-20. doi: 10.14715/cmb/2022.68.10.3
  27. Yaqub A, Nasir M,  Kamran M,  Majeed I,  Arif A.  Immunomodulation,  fish  health  and  resistance  to  staphylococcus  aureus  of  nile tilapia (Oreochromis niloticus) fed diet supplemented with zinc oxide nanoparticles and zinc acetate. Biological Trace Element Research. 2023;201(10):4912-4925. doi: 10.1007/s12011-023-03571-w
  28. Zhang L, Liu Z, Deng Y, He C, Liu W, Li X. The benefits of nanosized magnesium oxide in fish Megalobrama amblycephala: evidence in growth performance, redox defense, glucose metabolism, and magnesium homeostasis. Antioxidants (Basel). 2023;12(7):1350. doi: 10.3390/antiox12071350

Information about the authors:

Dmitry I Bulanin, Head of the laboratory of Biotechnology of Animal Raw Materials and Aquaculture, Orenburg State University, 13 Pobedy Ave, Orenburg, 460018; Postgraduate student of 1 year of study, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29 9 Yanvarya St., Orenburg, 460000, tel.: 8-951-030-98-62.

Marina S Mingazova, Cand. Sci. (Biology), Lecturer of the Department of Biotechnology of Animal Raw Materials and Aquaculture, Orenburg State University, 13 Pobedy Ave, Orenburg, 460018, tel: 8-922-853-24-46.

Elena P Miroshnikova, Dr. Sci. (Biology), Professor, Head of the Department of Biotechnology of Animal Raw Materials and Aquaculture, Orenburg State University, 13 Pobedy Ave, Orenburg, 460018, tel.: 8-987-862-98-86.

Yulia V Kilyakova, Cand. Sci. (Biology), Docent, Associate Professor, Department of Biotechnology of Animal Raw Materials and Aquaculture, Orenburg State University, 13 Pobedy Ave, Orenburg, 460018, tel.: 8-961-920-40-64.

Azamat E Arinzhanov, Cand. Sci. (Agriculture), Docent, Associate Professor, Department of Biotechnology of Animal Raw Materials and Aquaculture, Orenburg State University, 13 Pobedy Ave, Orenburg, 460018, tel.: 8-922-806-33-43.

The article was submitted 05.06.2025; approved after reviewing 01.08.2025; accepted for publication 15.09.2025.

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