Nechitailo KS, Sizova EA, Ryazanseva KV, Shoshin DE, Kholodilina TN.

Animal Husbandry and Fodder Production. 2025. Vol. 108. No 4. P. 73-85.

doi:10.33284/2658-3135-108-4-73

 

Original article

The effect of humic additive on the elemental homeostasis of broiler chickens

Ksenia S Nechitailo1, Elena A Sizova2, Kristina V Ryazantseva3, Daniil E Shoshin4,

Tatyana N Kholodilina5

1,2,3,4,5Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1k.nechit@mail.ru, https://orcid.org/0000-0002-8755-414X

2sizova.l78@yandex.ru, https://orcid.org/0000-0002-5125-5981

3reger94@bk.ru, https://orcid.org/0000-0001-5134-0396

4daniilshoshin@mail.ru, https://orcid.org/0000-0003-3086-681X

5xolodilina@rambler.ru, https://orcid.org/0000-0002-3946-8247

Abstract. The research was conducted to study the elemental status of broiler chickens of the Arbor-Aykres cross when a humic complex was introduced into the diet at a dosage of 0.01 ml per 1 kg of live weight. The elemental composition of the tissues was analyzed by inductively coupled plasma mass spectrometry. The results of the study showed that the introduction of the supplement led to significant changes in the elemental status of the chickens. In particular, the liver showed an increase in copper content by 14.3% (P≤0.01) and iron by 47.08% (P≤0.05), while the levels of manganese, cadmium and chromium decreased. In muscle tissue, there was an increase in iron fraction by 56.13% (P≤0.05), silicon by 48.4% (P≤0.05) and zinc by 6.53% (P≤0.05), while the levels of calcium and copper decreased. The feather sample showed an increase in concentration of iron and selenium with a decrease in chromium, magnesium and aluminum. The accumulation rate of elements such as copper, zinc and iron has increased, indicating an increase in their bioavailability. Thus, the humic additive helps to improve the elemental status of broiler chickens, increasing the bioavailability of essential trace elements and reducing the level of toxic metals. However, further research is needed to optimize the use of humic substances, including studying their effect on bone tissue and developing recommendations for use conditions. The data obtained confirm the prospects of using humic additive in poultry farming to increase productivity and product safety.

Keywords: elemental composition, broiler chickens, humic substances, humins, humic acids, microelements, macronutrients

Acknowledgments: the study was performed in accordance to the plan of research works for 2024-2026 FSBRI FRC BST RAS (No. FNWZ-2024-0002).

For citation: Nechitailo KS, Sizova EA, Ryazanseva KV, Shoshin DE, Kholodilina TN. The effect of humic additive on the elemental homeostasis of broiler chickens. Animal Husbandry and Fodder Production. 2025;108(4):73-85. (In Russ.). https://doi.org/10.33284/2658-3135-108-4-73

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Information about the authors:

Ksenia S Nechitailo, Dr Sci. (Biology), Senior Researcher of the Department of Physiology, Biochemistry and Morphology of Animals, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000, tel.: 89058935599.

Elena A Sizova, Dr Sci. (Biology), Chief Researcher, Head of the Department of Physiology, Biochemistry and Morphology of Animals, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000, tel.: 89123449907.

Kristina V Ryazantseva, Dr Sci. (Biology), Researcher of the Department of Physiology, Biochemistry and Morphology of Animals, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000, tel.: 89867759545.

Daniil E Shoshin, post-graduate student, Junior Researcher of the Department of Physiology, Biochemistry and Morphology of Animals, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000, tel.: 89659325367.

Tatyana N Kholodilina, Cand. Sci. (Agriculture), Head of the Center «Testing Center» of the Central Common Use Center, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000, tel.: 8(3532)77-39-97.

The article was submitted 23.09.2025; approved after reviewing 23.10.2025; accepted for publication 15.12.2025.

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