Tuzikov AP, Miroshnikov SA, Kvan OV, Bykov AV.

Animal Husbandry and Fodder Production. 2026. Vol. 109. No. 2. Р. 46-58.

 

doi: 10.33284/2658-3135-109-2-46

 

Original article

Assessment of the effect of a mixture of ultrafine particles on the elemental status of broiler chickens

 

Alexey P Tuzikov1, Sergey A Miroshnikov2, Olga V Kvan3, Artem V Bykov4

1,2,3,4Orenburg State University named after VА Bondarenko, Orenburg, Russia

1sergey_ru01@mail.ru

2 rector_osu@mail.osu.ru, https://orcid.org/0000-0003-1173-1952

3kwan111@yandex.ru, https://orcid.org/0000-0003-0561-7002

4artem19782@yandex.ru, https://orcid.org/0000-0003-4844-4631

 

Abstract. Modern industrial poultry farming requires a revision of approaches to mineral nutrition due to the high productivity of crosses and low bioavailability of traditional inorganic salts of trace elements. The use of ultrafine metal particles (UMP) is promising; however, their comprehensive effect on the mineral status in the poultry body has not been sufficiently studied. The aim of the study was to evaluate the effect of a mixture of UMP of Fe, Cu, Zn and Mn on a carbon substrate on the growth and elemental status of broiler chickens. Studies have shown that the additional administration of the UMP mixture contributed to the redistribution of minerals in the tissues of the studied bird. In the muscle tissue of the experimental bird, a significant increase in calcium by 3.8 times (P≤0.001), selenium by 24.6% (P≤0.05) and chromium by 27.2% (P≤0.05) was detected against the background of a decrease in sodium by 14.7% (P≤0.05). Significant accumulation of nickel, iodine, chromium and cobalt (P≤0.001) was noted in the body of the bird. An important effect was a decrease in the level of toxic elements by 43.0-52.0% (P≤0.01). The data obtained indicate that the mixture of UMP elements helps to reduce the content of toxic elements in tissues and change the distribution of a number of macro- and microelements in the body of broiler chickens.

Keywords: broiler chickens, feeding, ultrafine particles, mineral metabolism, elemental composition

Acknowledgements: the work was carried out with the financial support of a grant for major scientific projects in priority areas of scientific and technological development (No. 075-15-2024-550).

For citation: Tuzikov AP, Miroshnikov SA, Kvan OV, Bykov AV. Assessment of the effect of a mixture of ultrafine particles on the elemental status of broiler chickens. Animal Husbandry and Fodder Production. 2026;109(2):46-58. (In Russ.). https://doi.org/10.33284/2658-3135-109-2-46

 

References

  1. Miroshnikova EP, Kvan OV, Rusakova EA, Sheyda EV, Rusakova EA. Effect of Quercus cortex extract and ultrafine particles of Fe and Cu on the exchange of chemical elements in body of broiler chickens. Animal Husbandry and Fodder Production. 2020;103(3):24-35. doi: 10.33284/2658-3135-103-3-24
  2. Egorov I, et al. A Methodological Guide to Feeding Farm Poultry. Edited by Fisinin. V, Yegorova IA. Sergiev Posad: VNITIP; 2021:360 p.
  3. Sizova EA, Miroshnikov SA, Lebedev SV, Levakhin YuI, Babicheva IA, Kosilov VI. Comparative tests of various sources of microelements in feeding chicken-broilers. Agricultural Biology. 2018;53(2):393-403. doi: 10.15389/agrobiology.2018.2.393rus doi: 10.15389/agrobiology.2018.2.393eng
  4. Miroshnikova EP, Ryazanov VA, Miroshnikov IS, Tuzikov AP. Comparative analysis of the elemental composition of blood and muscle tissue in broiler chickens when introducing iron and zinc nanocomposites into the diet. Achievements of Science and Technology in Agro-Industrial Complex. 2025;39(8):82-88. doi: 10.53859/02352451_2025_39_8_82
  5. Amlan P, Lalhriatpuii M. Progress and prospect of essential mineral nanoparticles in poultry nutrition and feeding – a review. Biol Trace Elem Res. 2020;197(1):233-253. doi: 10.1007/s12011-019-01959-1
  6. Ao T, Pierce JL, Power R, Pescatore AJ, Cantor AH, Dawson KA, Ford MJ. Effects of feeding different forms of zinc and copper on the performance and tissue mineral content of chicks. Poult Sci. 2009;88(10):2171-217 doi: 10.3382/ps.2009-00117
  7. Aslam MF, Frazer DM, Faria N, Bruggraber SF, Wilkins SJ, Mirciov C, Powell JJ, Anderson GJ, Pereira DI. Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice. FASEB 2014;28(8):3671-3678. doi: 10.1096/fj.14-251520
  8. Bhanja S, Verma S. Prospects of Nano minerals in poultry nutrition. Indian Journal of Poultry Science. 2021;56(1):1-8. doi: 10.5958/0974-8180.2021.00006.4
  9. Birt DF, Julius AD, Runice CE, White LT, Lawson T, Pour PM. Enhancement of BOP-induced pancreatic carcinogenesis in selenium-fed Syrian golden hamsters under specific dietary conditions. Nutrition and Cancer. 1988;11(1):21-33. doi: 10.1080/01635588809513966
  10. Braun V, Killmann H. Bacterial solutions to the iron-supply problem. Trends Biochem Sci. 1999;24(3):104-109. doi: 10.1016/s0968-0004(99)01359-6
  11. Choi JW, Kim SK. Relationships of lead, copper, zinc, and cadmium levels versus hematopoiesis and Iron parameters in healthy adolescents. Annals of Clinical and Laboratory Science. 2005;35(4):428-434.
  12. Delaby C, Pilard N, Goncalves AS, Beaumont C, Canonne-Hergaux F. Presence of the iron exporter ferroportin at the plasma membrane of macrophages is enhanced by iron loading and down-regulated by hepcidin. Blood. 2005;106(12): 3979-3984, 2005. doi: 10.1182/blood-2005-06-2398
  13. El-Maddawy ZK,  El-sawy  A-EF,  Ashoura  NR,  Aboelenin SM, Soliman MM, Ellakany HF, Elbestawy AR, El-Shall NA. Use of zinc oxide nanoparticles as anticoccidial agents in broiler chickens along with its impact on growth performance, antioxidant status, and hematobiochemical profile. Life. 2022;12(1):74. doi: 10.3390/LIFE12010074
  14. Fung EB, Ritchie LD, Woodhouse LR, Roehl R, King JC. Zinc absorption in women during pregnancy and lactation: a longitudinal study. Am J Clin Nutr. 1997;66(1):80-88. doi: 10.1093/ajcn/66.1.80
  15. Gopi M, Pearlin B, Kumar RD. Role of nanoparticles in animal and poultry nutrition: modes of action and applications in formulating feed additives and food processing. International Journal of pharmacology. 2017;13(7):724-731. doi: 10.3923/ijp.2017.724.731
  16. Gubajdullina IZ, Gavrish IA, Lebedev SV. Effect of metallic nanoparticles on exchange of chemical elements in broiler chikens. IOP Conference Series: Earth and Environmental Science. 2019;341(1):012169. doi:10.1088/1755-1315/341/1/012169
  17. Hassan S, Hassan  Fu,  Rehman MSu. Nano-particles of trace minerals in poultry nutrition:  potential  applications and future prospects. Biol Trace Elem Res. 2020;195:591-612. doi: 10.1007/s12011-019-01862-9
  18. Jankowski J, Katarzyna O, Stepniowska A, Zdunczyk ZZ, et al. The effect of the source and dose of manganese on the performance, digestibility and distribution of selected minerals, redox and immune status of turkeys. Poultry Science. 2019;98(3):1379-1389. doi: 10.3382/ps/pey467
  19. Khot LR, Sankaran S, Maja JM, et al. Applications of nanomaterials in agricultural production and crop protection: a review. Crop Protection. 2012;35:64-70 doi: 10.1016/j.cropro.2012.01.007
  20. Košir D, Vrečer    The  performance  of  HPMC  matrix  tablets  using  various  agglomeration manufacturing processes. Drug Dev Ind Pharm. 2017;43(2):329-337. doi: 10.1080/03639045.2016.1249374
  21. Lebedev S, Sheida E, Vershinina I. Use of chromium nanoparticles as a protector of digestive enzymes and biochemical parameters for various sources of fat in the diet of calves. AIMS Agriculture and Food. 2021;6(1):14-31. doi: 10.3934/agrfood.2021002
  22. Matuszewski A, Łukasiewicz M,  Niemiec    Calcium  and  phosphorus  and their   nanoparticle   forms  in  poultry  nutrition.  World's  Poult  Sci  J.  2020;76(2):328-345. doi: 10.1080/00439339.2020.1746221
  23. Miroshnikov S, Yausheva E, Sizova E, Miroshnikova E. Comparative assessment of effect of copper nano- and microparticles in chicken. Orient J Chem. 2015;31(4):2327-2336. doi:13005/ojc/310461
  24. Ouyang Z, Ren P, Zheng D, Huang L, Wei T, Yang C, Kong X, Yin Y, He S, He Q. Hydrothermal synthesis of a new porous zinc oxide and its antimicrobial evaluation in weanling piglets. Livestock Sci. 2021;248:104499. doi:1016/j.livsci.2021.104499
  25. Patra A, Lalhriatpuii M. Progress and prospect of essential mineral nanoparticles in  poultry nutrition and feeding—a review. Biol Trace Elem Res. 2020;197:233-253. doi: 10.1007/s12011-019-01959-1
  26. Sahnen F, Kamps JP, Langer K. Conversion of indomethacin nanosuspensions into solid dosage forms via fluid bed granulation and compaction. Eur J Pharm Biopharm. 2020;154:89-97. doi: 10.1016/j.ejpb.2020.06.020
  27. Sizova E, Miroshnikov S, Yausheva E, Polyakova V. Assessment of morphological and functional changes in organs of rats after intramuscular introduction of iron nanoparticles and their agglomerates. Biomed Res Int. 2015; 3:243173. doi: 10.1155/2015/243173
  28. Skřivan M, Skřivanová V, Marounek M. Effects of dietary zinc, iron, and copper in layer feed on distribution of these elements in eggs, liver, excreta, soil, and herbage. Poultry Science. 2005;84(10):1570-1575. doi: 10.1093/ps/84.10.1570
  29. Szuba-Trznadel A, Rząsa A, Hikawczuk T, Fuchs B. Effect of zinc source and level on growth performance and zinc status of weaned piglets. Animals. 2021;11(7):2030. doi: 10.3390/ani11072030
  30. Tian X, Chen H, Ren D, Yang X. Selenium nanoparticles driving innovations across the food chain: applicationa in food systems, feed supplementation, and sustainable agriculture. Journal of Agricultural and Food Chemistry. 2026;74(7):5941-5961. doi: 10.1021/acs.jafc.5c16705
  31. Wang Y, Ou YL, Liu YQ, Xie Q, Liu QF, Wu Q, Fan TQ, Yan LL, Wang JY.Correlations of trace element levels in the diet, blood, urine, and feces in the Chinese male. Biol Trace Elem Res. 2012;145(2):127-135. doi: 10.1007/s12011-011-9177-8
  32. Yusof HM, Mohamad R, Zaidan UH, Rahman NAA. Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review. J Anim Sci Biotechnol. 2019;10:57. doi: 10.1186/s40104-019-0368-z
  33. Zulficar S, Riasat M, Majeed S, Rehman Z. Investigation of the influence of dietary iron oxide nanoparticles on immunity and oxidative stress markers in Gallus gallus domesticus. Pakistan Journal of Zoological Sciences. 2025;1(3):1-8. doi: 10.65139/xrztbz33

Information about the authors:

Alexey P Tuzikov,  Junior Researcher, Orenburg State University named after VА Bondarenko, 13 Pobedy Ave., Orenburg, 460018, tel.: 8 (3532) 77-67-70.

Sergey A Miroshnikov, Dr. Sci. (Biology), Professor, Corresponding Member of the Russian Academy of Sciences, Rector, Orenburg State University named after VA Bondarenko, 13 Pobedy Ave, Orenburg, 460018, tel.: 8 (3532)77-67-70.

Olga V Kvan, Dr. Sci.  (Biology),  Professor  of  the  Department, named after VА Bondarenko, 13 Pobedy ave., Orenburg, 460018, tel.: 89225485657.

Artem V Bykov, Dr. Sci. (Biology), docent of the Department, Orenburg State University named after VA Bondarenko, 13 Pobedy Ave, Orenburg, 460018, tel.: 8-922-869-74-09.

The article was submitted 20.04.2026; approved after reviewing 26.05.2026; accepted for publication 15.06.2026.

Download