Tishin AA, Silin DA.

Animal Husbandry and Fodder Production. 2026. Vol. 109. No. 3. Р. 195-211.

doi: 10.33284/2658-3135-109-3-195

Original article

 

The effect of probiotics in combination with microparticles of microelements and microcrystalline cellulose on the zootechnical indicators of broiler chickens

 

Alexander A Tishin 1, Dmitriy A Silin2

1,2Orenburg State University named after VА Bondarenko

1alex.vet7787@yandex.ru, https://orcid.org/0009-0005-3808-4275

2dasilin@mail.ru, https://orcid.org/0000-0001-6438-139X

 

Abstract. The paper presents the results of an experimental study aimed at assessing the effect of a complex feed additive, which includes a probiotic preparation (Lactobacillus delbrueckii subsp. bulgaricus 40 mg/kg and Streptococcus thermophilus 40 mg/kg), metal microparticles (MMP) (Fe, Cu, Zn), and microcrystalline cellulose (MCC), on the productive traits, digestibility of nutrients, and chemical composition of the body pn broiler chickens of the “Ross 308” cross. The experiment was conducted on 180 broiler chickens for a period of 8-35 days, of which 6 groups were formed: a control group and five experimental groups receiving various combinations of the studied components. It was found that the greatest positive effect was observed in the group, which received a full range of supplements (MCC + probiotic + MMP Cu, Fe, Zn). In this group, there was a decrease in feed costs per 1 kg of gain by 8%, an increase in the digestibility of crude protein by 6.1% in the initial period and by 5,3% in the growth period, as well as an increase in body weight by 5.6% by the age of 35 days compared with the control. The chemical composition of the body was characterized by a maximum accumulation of dry matter (32.7%) and protein (22.1%) with a moderate fat content, which meets the quality criteria of meat products. The transformation coefficient of the feed protein in the body was 41.9%, the energy conversion reached 37.3%. The results obtained indicate the synergistic effect of the combined use of probiotics, micro metal particles and microcrystalline cellulose, which optimizes digestive processes, increases the bioavailability of nutrients and the effectiveness of their transformation into meat products. A differentiated reaction to the introduction of monovariants of trace elements was revealed, confirming the advantage of an integrated approach.

Keywords: broiler chickens, feeding, digestibility, diet, productivity, microcrystalline cellulose, probiotic, microparticles

Acknowledgments: the research was carried out with the support of a grant for major scientific projects in priority areas of scientific and technological development (No. 075-15-2024-550).

For citation: Tishin AA, Silin DA. The effect of probiotics in combination with microparticles of microelements and microcrystalline cellulose on the zootechnical indicators of broiler chickens. Animal Husbandry and Fodder Production. 2026;109(3):195-211. (In Russ.). https://doi.org/10.33284/2658-3135-109-3-195

References

  1. State Standard 31640-2012. Feeds. Methods for determination of dry matter content. Implementation date 2013-07-01. Moscow: Standartinform; 2012:12 p.
  2. State Standard 32044.1-2012. Feeds, compound feeds, feed raw materials. Determination of mass fraction of nitrogen and calculation of mass fraction of crude protein. Part 1. Kjeldahl method. Implementation date 2014-07-01. Moscow: Standartinform; 2014:15 p.
  3. State Standard 13496.15-97. Forages, compound feeds, raw materials for compound feeds. Methods for determination of crude fat content. Implementation date 1999-01-01. Moscow: IPK Publishing House of Standards; 1998:12 p.
  4. State Standard R 51479-99. Meat and meat products. Method for determination of moisture content. Implementation date 2001-01-01. Moscow: IPK Publishing House of Standards; 2000:6 p.
  5. State Standard 23042-86. Meat and meat products. Methods for determination of fat. Implementation date 1988-01-01. Moscow: IPK Publishing House of Standards; 2010:6 p.
  6. State Standard 25011-81. Meat and meat products. Methods for determination of protein. Implementation date 1983-01-01. Moscow: IPK Publishing House of Standards; 2010:8 p.
  7. State Standard R 53642-2009. Meat and meat products. Method for determination of total ash content. Implementation date 2011-01-01. Moscow: Standartinform; 2010:12 p.
  8. Ivanischeva AP, Sizova EA, Nechitailo KS. Digestibility of nutrients after using an organic mineral supplement in the diet of broiler chickens. Animal Husbandry and Fodder Production. 2021;104(4):22-31.https://doi.org/10.33284/2658-3135-104-4-22
  9. Kvan OV, Sheida EV, Miroshnikova EP, Bykov AV, Baranova OV, Kuryakova TA. Study of the effect of ultrafine particles on microbiocenosis of the blind intestine of broiler chickens. Problems of Biological Medical and Pharmaceutical Chemistry. 2024;27(11):56-64. doi: 10.29296/25877313-2024-11-07
  10. Egorov IA et al. The methodology of scientific and industrial research on poultry feeding. Molecular genetic methods for the determination of intestinal microflora. Sergiev Posad: Ves’ Sergiev Posad; 2013:51 p.
  11. Egorov IA, Lenkova ТN et al.; under general ed. VI Fisinin, IA Egorov. Methodical manual on feeding of agricultural poultry Sergiev Posad: VNITIP, 2021:360 p.
  12. Nechitailo KS, Sizova EA. Effect of a multi-enzyme feed additive on productivity, digestibility and body chemistry of broiler chickens. Animal Husbandry and Fodder Production. 2021;104(4):148-157. https://doi.org/10.33284/2658-3135-104-4-148
  13. Sizova EA, Nechitailo KS, Ivanischeva AP, Ryabov NI. The prospects of using ultra-dispersed forms of metals in animal feeding. Animal Husbandry and Fodder Production. 2020;103(3):177-189. doi: 10.33284/2658-3135-103-3-177
  14. Fisinin VI. Poultry farming in Russia – strategy of innovative development. Moscow: Russian Academy of Agricultural Sciences; 2009:147 p.
  15. Alqahtani AN. Impact of dietary zinc nanoparticles and probiotics on broiler health and productivity. J Adv Vet Anim Res. 2025;12(1):19-32. doi: 10.5455/javar.2025.l868
  16. Al-Ruwad SH, Attia AI,  Abdel Monem UM,  Abdel-Maksoud  A, Thagfan FA, Alqahtani HA, Alkahtani AM, Salah AS, Reda FM. Dietary supplementation with copper nanoparticles enhances broiler performance by improving growth, immunity, digestive enzymes, and gut microbiota. Poult Sci. 2024;103(10):104026. doi: 10.1016/j.psj.2024.104026
  17. Becker KW, Skaar EP. Metal limitation and toxicity at the interface between host and pathogen. FEMS Microbiol Rev. 2014;38(6):1235-49. doi: 10.1111/1574-6976.12087
  18. Bhanja SK, Verma SVS. Prospects of nano minerals in poultry nutrition. Indian Journal of Poultry Science. 2021;56(1):1-8. doi: 10.5958/0974-8180.2021.00006.4
  19. Broom LJ, Monteiro A, Piñon A. Recent advances in understanding the influence of zinc, copper, and manganese on the gastrointestinal environment of pigs and poultry. Animals (Basel). 2021;11(5):1276. doi: 10.3390/ani11051276
  20. Chen C, Qu M, Liang H, Ouyang K, Xiong Z, Zheng Y, Yan Q, Xu L. Gastrointestinal digestibility insights of different levels of coated complex trace minerals supplementation on growth performance of yellow-feathered broilers. Front Vet Sci. 2022;9:982699. doi: 10.3389/fvets.2022.982699
  21. Choi EK, Aring L, Das NK, Solanki S, Inohara N, Iwase S, Samuelson LC, Shah YM, Seo YA. Impact of dietary manganese on experimental colitis in mice. FASEB J. 2020;34(2):2929-2943. doi: 10.1096/fj.201902396R
  22. Dalecki AG, Crawford CL, Wolschendorf F. Copper and Antibiotics: Discovery, Modes of Action, and  Opportunities  for  Medicinal    Adv  Microb  Physiol.  2017;70:193-260. doi: 10.1016/bs.ampbs.2017.01.007
  23. Guo Z, Zhang L, Lu W, Fang Z, Zhang C, Zhang Y, Chen L. Effect of early feeding of Limosilactobacillus fermentum on growth performance and intestinal development in broilers. Poult Sci. 2026;105(1):106040. doi: 10.1016/j.psj.2025.106040
  24. Hooda S, Metzler-Zebeli BU, Vasanthan T, Zijlstra RT. Effects of viscosity and fermentability of dietary fibre on nutrient digestibility and digesta characteristics in ileal-cannulated grower pigs. Br J Nutr. 2011;106(5):664- doi: 10.1017/S0007114511000985
  25. Ismail H, Ibrahim D, El Sayed S, Wahdan A, El-Tarabili RM, Rizk El-Ghareeb W, Abdullah Alhawas B, Alahmad BAY, Abdel-Raheem SM, El-Hamid MIA. Prospective application of nanoencapsulated Bacillus amyloliquefaciens on broiler chickens' performance and gut health with efficacy against Campylobacter jejuni colonization. Animals (Basel). 2023;13(5):775. doi: 10.3390/ani13050775
  26. Jha R, Berrocoso JD. Review: Dietary fiber utilization and its effects on physiological functions and gut health of swine. Animal. 2015;9(9):1441-1452. doi: 10.1017/S1751731115000919
  27. Jha R, Berrocoso JFD. Dietary fiber and protein fermentation in the intestine of swine and their interactive effects on gut health and on the environment: a review. Anim Feed Sci Technol. 2016;212:18- doi: 10.1016/j.anifeedsci.2015.12.002
  28. Knight LC, Wang M, Donovan SM, Dilger RN. Early-life iron deficiency and subsequent repletion alters development of the colonic microbiota in the pig. Front Nutr. 2019;6:120. doi: 10.3389/fnut.2019.00120
  29. Liu C, Ma N, Feng Y, Zhou M, Li H, Zhang X, et al. From probiotics to postbiotics: concepts and applications. Anim Res One Health. 2023;1(1):92- doi: 10.1002/aro2.7
  30. Liu ZH, Lu L, Wang RL, Lei HL, Li SF, Zhang LY, Luo XG. Effects of supplemental zinc source and level on antioxidant ability and fat metabolism-related enzymes of broilers. Poult Sci. 2015;94(11):2686-94. doi: 10.3382/ps/pev251
  31. Pieper R, Vahjen W, Neumann K, Van Kessel AG, Zentek J. Dose-dependent effects of dietary zinc oxide on bacterial communities and metabolic profiles in the ileum of weaned pigs. J Anim Physiol Anim Nutr (Berl). 2012;96(5):825-33. doi: 10.1111/j.1439-0396.2011.01231.x
  32. Pontin KP, Borges KA, Furian TQ, Carvalho D, Wilsmann DE, Cardoso HRP, Alves AK, Chitolina GZ, Salle CTP, Moraes HLS, do Nascimento VP. Antimicrobial activity of copper surfaces against biofilm formation by Salmonella Enteritidis and its potential application in the poultry industry. Food Microbiol. 2021;94:103645. doi: 10.1016/j.fm.2020.103645
  33. Satessa GD, Kjeldsen NJ, Mansouryar M, Hansen HH, Bache JK, Nielsen MO. Effects of alternative feed additives to medicinal zinc oxide on productivity, diarrhoea incidence and gut development in weaned piglets. Animal. 2020;14(8):1638-1646. doi: 10.1017/S1751731120000154
  34. Singh P, Prasad S. A review on iron, zinc and calcium biological significance and factors affecting  their  absorption  and bioavailability. J Food Compos Anal. 2023;123:105529. doi: 10.1016/j.jfca.2023.105529
  35. Tang X, Liu X and Liu H. Effects of dietary probiotic (Bacillus subtilis) supplementation on carcass traits, meat quality, amino acid, and fatty acid profile of broiler chickens. Front Vet Sci. 2021;8:767802. doi: 10.3389/fvets.2021.767802
  36. Tejeda OJ, Kim WK. Role of dietary fiber in poultry nutrition. Animals (Basel). 2021;11(2):461. doi: 10.3390/ani11020461
  37. Tejeda OJ, Kim WK. The effects of cellulose and soybean hulls as sources of dietary fiber on the growth performance, organ growth, gut histomorphology, and nutrient digestibility of broiler chickens. Poult Sci. 2020;99(12):6828-6836. doi: 10.1016/j.psj.2020.08.081
  38. Zainuddin Z et al. Effects of dietary bacillus coagulans D3372 supplementation as probiotics on broiler performance, ileal microflora, meat quality, nutrient retention, and metabolizable energy. Advances in Animal and Veterinary Sciences. 2020;8(1):115-123. doi: 10.17582/JOURNAL.AAVS/2020/8.1.115.123
  39. Zijlstra RT, Jha R, Woodward AD, Fouhse J, van Kempen, TATG. Invited review: starch and fiber properties affect their kinetics of digestion and thereby digestive physiology in pigs. J Anim Sci. 2012;90(E-suppl. 4):49-58. doi: 2527/jas.53718

 

Information about the authors:

Alexander A Tishin, Research Assistant at the Institute of Bioelementology, Orenburg State University named after VА Bondarenko, Russia, Orenburg, 13 Pobedy Ave., 460018, tel.: 89878886665.

Dmitriy A Silin, Cand. Sci. (Biology), Research Assistant at the Institute of Bioelementology, Orenburg State University named after VА Bondarenko, Russia, Orenburg, 13 Pobedy Ave., 460018, tel.: 89534575189.

The article was submitted 20.07.2026; approved after reviewing 25.08.2026; accepted for publication 14.09.2026.

Download