Vladimir Yu Babenkov, Nadezhda V Chimidova, Arslang I Khakhlinov, Altana V Ubushieva, Vasiliy I Manjiev

Animal Husbandry and Fodder Production. 2023. Vol. 106, no 1. Р. 67-76.

 

doi:10.33284/2658-3135-106-1-67

 

Original article

The role of reproductive biotechnologies in the reproduction and preservation of the

gene pool of rare and endangered breeds of cattle

 

Vladimir Yu Babenkov1, Nadezhda V Chimidova2, Arslang I Khakhlinov3, Altana V Ubushieva4,

Vasiliy I Manjiev5

1,2,3,4,5Kalmyk State University named after B.B. Gorodovikov, Elista, Russia

1v.babenkov@mail.ru, https://orcid.org/0000-0003-1061-2281

2nadezhdatchimidova@yandex.ru, https://orcid.org/0000-0003-3043-091X

3arspeople@mail.ru,  https://orcid.org/0000-0002-1373-0243

4ameli-altanas@mail.ru, https://orcid.org/0000-0002-9916-7972

5vasyaman33@gmail.com, https://orcid.org/0000-0002-5545-470X

 

Abstract. The current situation with domestic breeds, associated with the reduction of breed diversity in dairy and beef cattle breeding in recent years requires urgent measures to be taken to preserve their gene pool. Today, populations of endangered breeds can be restored through the creation of gene pool breeding farms and the development of assisted reproductive technologies (ART). In the latter case, cryostorages of semen and embryos are created in a frozen state, where genetic resources are isolated from the evolutionary process and are preserved in their original form indefinitely. The aim of the research was to determine the effectiveness of the application of methods of reproductive biotechnologies for the conservation of gene pool breeds on the example of the endangered Red Gorbatov breed with high adaptive qualities in the arid region of Kalmykia. The studies were carried out in 2017-2018 and 2022 in JSC "Ababkovsky" of Nizhny Novgorod region, "Batu plus" Peasant Farm and Regional Research and Production Center for reproduction in farm animals of the Federal State Budgetary Educational Institution of Higher Education "Kalmyk State University named after B.B. Gorodovikov" of Republic of Kalmykia. During the experiments, the embryonic productivity of cows of the Red Gorbatov breed, their fertility through interbreed embryo transfer and the ability to acclimatize transplant calves with passive immunity of surrogate mothers in an arid region were studied. The experiments used applied methods of biotechnology in vivo: induction of poliovulation, artificial insemination, non-surgical extraction, cryopreservation and embryo transfer. 23 washes resulted in 223 viable embryos, or 9.7 embryos on average per donor. Suitable embryos were cryopreserved. In the course of the experiments, embryos of the Red Gorbatov breed after deconservation were transplanted into recipient heifers of the Kalmyk beef breed to transfer passive immunity to offspring. A total of 117 embryos were transferred to 117 recipients, of which 73 were pregnant. Thus, the level of engraftment of embryos was 62.4%. High rates of embryo productivity and survival of embryos indicate high fertility of mothers of embryos.

Keywords: cows, heifers, Red Gorbatov breed, Kalmyk beef breed, breeding, biotechnology, embryos, transplantation, poliovulation, egg

Acknowledgments: the work was performed in accordance to the plan of research works for 2022-2024 by Kalmyk State University (No. 075-03-2022-119/1).

For citation: Babenkov VYu, Chimidova NV, Khakhlinov AI, Ubushieva AV, Manjiev VI. The role of reproductive biotechnologies in the reproduction and preservation of the gene pool of rare and endangered breeds of cattle. Animal Husbandry and Fodder Production. 2023;106(1):67-76. (In Russ.). https://doi.org/10.33284/2658-3135-106-1-67

 

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Information about the authors:

Vladimir Yu Babenkov, Dr. Sci (Biology), Head of the Laboratory of Genomics and Biochemistry of the RNPC for the Reproduction of farm animals, Leading researcher of the laboratory "Features of the organization of the genome of meat breeds associated with high adaptive and productive potential based on highly polymorphic genetic markers", Kalmyk State University named after B.B. Gorodovikov, 5 microdistrict, building 4, KalmSU building, Republic of Kalmykia, Elista, 358011, cell: 89803781833.

Nadezhda V Chimidova, Cand. Sci. (Biology), Head of the laboratory "Features of the organization of the genome of meat breeds associated with high adaptive and productive potential based on highly polymorphic genetic markers", Associate Professor of the Department of Biotechnology and Animal Husbandry of the Faculty of Agriculture, Kalmyk State University named after B.B. Gorodovikov, 5 microdistrict, building 4, KalmSU building, Republic of Kalmykia, Elista, 358011, cell: 89374620111.

Arslang I Khakhlinov, Director of the Regional Research and Production Center for the Reproduction of Farm Animals, researcher of the laboratory "Features of the organization of the genome of meat breeds associated with high adaptive and productive potential based on highly polymorphic genetic markers", Kalmyk State University named after B.B. Gorodovikov, 5 microdistrict, building 4, KalmSU building, Republic of Kalmykia, Elista, 358011, cell: 89615463555.

Altana V Ubushieva, Researcher of the laboratory "Features of the organization of the genome of meat breeds associated with high adaptive and productive potential based on highly polymorphic genetic markers", senior lecturer Department of Biotechnology and Animal Husbandry of the Faculty of Agriculture, Kalmyk State University named after B.B. Gorodovikov, 5 microdistrict, building 4, KalmSU building, Republic of Kalmykia, Elista, 358011, cell: 89996701448.

Vasily I Manjiev, Research Engineer of the laboratory "Features of the organization of the genome of meat breeds associated with high adaptive and productive potential based on highly polymorphic genetic markers", Kalmyk State University named after B.B. Gorodovikov, 5 microdistrict, building 4, KalmSU building, Republic of Kalmykia, Elista, 358011, cell: 89272832088.

 

The article was submitted 30.01.2023; approved after reviewing 13.02.2023; accepted for publication 20.03.2023.

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